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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in several tumors, which leads to chromosome instability, and therefore may well lead to tumorigenesis. This protein was identified to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a crucial job in endorsing mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have been described. Two supplemental splice variants ended up claimed but comprehensive nucleotide sequence facts just isn't yet accessible.
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Blocking peptides are peptides that bind specifically to the goal antibody and block antibody binding. These peptide ordinarily includes the epitope identified by the antibody.
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This guide outlines more probable optimization of cellular and immunoassay parameters to make certain the very best final results are...
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This tutorial outlines more possible optimization of cellular and immunoassay parameters to make certain the very best results are...
Antibodies bound to the blocking peptide not bind to your epitope about the concentrate on protein. By comparing the staining in the blocked antibody compared to the antibody on your own, you can see which staining is precise.
Concentrate on Information and facts Cyclin E belongs for the very conserved cyclin relatives, whose members are characterized by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinctive cyclins show unique expression and degradation patterns which contribute to your temporal coordination of each and every mitotic celebration. Cyclin E types a fancy with and capabilities as being a regulatory subunit of CDK2, whose action is needed for mobile cycle G1/S changeover. This protein accumulates for the G1-S stage boundary and is particularly degraded as cells development as a result of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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